LOCUS       40924_13610        5214 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cre expression vector pCS2-olCre DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Kishimoto K.
  TITLE     Two useful site-specific recombination system VCre/VloxP and 
            SCre/SloxP in Medaka
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Kishimoto K, Kinoshita M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2015) Contact:Kenta Kishimoto Kyoto University, 
            Graduate School of Agriculture; Kitashirakawa Oiwaketyo, Sakyo, 
            Kyoto 606-8502, Japan URL :http://www.kais.kyoto-u.ac.jp/english/
REFERENCE   3  (bases 1 to 5214)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2015) Contact:Kenta Kishimoto Kyoto University, Graduate 
            School of Agriculture"; volume: " Kitashirakawa Oiwaketyo, Sakyo, 
            Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.kais.kyoto-u.ac.jp/english"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    127..153
                     /gene="oCre"
                     /note="NLS; PPPKRPRLD"
     CDS             190..1218
                     /label=Cre
                     /note="site-specific recombinase"
     promoter        complement(1241..1258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(1263..1397)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1510..1528)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1549..1565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1573..1589)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1597..1627)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1642..1663)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1951..2539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2713..3570)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3571..3675)
                     /label=AmpR promoter
     rep_origin      complement(3701..4156)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4230..5214
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca ccatggcgga ggccctcgag
      121 gctagccctc ctcctaagag acctagactg gactctagaa tggcgcgccc cggaggtagc
      181 ggtggaacta tgagcaacct gctgaccgtg caccagaacc tgcccgccct gcccgtggac
      241 gccaccagcg acgaggtgag gaagaacctg atggacatgt tcagggacag gcaggccttc
      301 agcgagcaca cctggaagat gctgctgagc gtgtgcagga gctgggccgc ctggtgcaag
      361 ctgaacaaca ggaagtggtt ccccgccgag cccgaggacg tgagggacta cctgctgtac
      421 ctgcaggcca ggggcctggc cgtgaagacc atccagcagc acctgggcca gctgaacatg
      481 ctgcacagga ggagcggcct gcccaggccc agcgacagca acgccgtgag cctggtgatg
      541 aggaggatca ggaaggagaa cgtggacgcc ggcgagaggg ccaagcaggc cctggccttc
      601 gagaggaccg acttcgacca ggtgaggagc ctgatggaga acagcgacag gtgccaggac
      661 atcaggaacc tggccttcct gggcatcgcc tacaacaccc tgctgaggat cgccgagatc
      721 gccaggatca gggtgaagga catcagcagg accgacggcg gcaggatgct gatccacatc
      781 ggcaggacca agaccctggt gagcaccgcc ggcgtggaga aggccctgag cctgggcgtg
      841 accaagctgg tggagaggtg gatcagcgtg agcggcgtgg ccgacgaccc caacaactac
      901 ctgttctgca gggtgaggaa gaacggcgtg gccgccccca gcgccaccag ccagctgagc
      961 accagggccc tggagggcat cttcgaggcc acccacaggc tgatctacgg cgccaaggac
     1021 gacagcggcc agaggtacct ggcctggagc ggccacagcg ccagggtggg cgccgccagg
     1081 gacatggcca gggccggcgt gagcatcccc gagatcatgc aggccggcgg ctggaccaac
     1141 gtgaacatcg tgatgaacta catcaggaac ctggacagcg agaccggcgc tatggtgagg
     1201 ctgctggagg acggcgactg agtcgagcat gcatctagaa ctatagtgag tcgtattacg
     1261 tagatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     1321 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     1381 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     1441 aggtgtggga ggttttttaa ttcgcggccg cggcgccaat gcattgggcc cggtacccag
     1501 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     1561 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1621 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1681 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1741 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     2401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3661 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     3721 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3781 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3841 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3901 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3961 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     4021 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     4081 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     4141 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4201 gtgcgggcct cttcgctatt acgccagtcg accatagcca attcaatatg gcgtatatgg
     4261 actcatgcca attcaatatg gtggatctgg acctgtgcca attcaatatg gcgtatatgg
     4321 actcgtgcca attcaatatg gtggatctgg accccagcca attcaatatg gcggacttgg
     4381 caccatgcca attcaatatg gcggacttgg cactgtgcca actggggagg ggtctacttg
     4441 gcacggtgcc aagtttgagg aggggtcttg gccctgtgcc aagtccgcca tattgaattg
     4501 gcatggtgcc aataatggcg gccatattgg ctatatgcca ggatcaatat ataggcaata
     4561 tccaatatgg ccctatgcca atatggctat tggccaggtt caatactatg tattggccct
     4621 atgccatata gtattccata tatgggtttt cctattgacg tagatagccc ctcccaatgg
     4681 gcggtcccat ataccatata tggggcttcc taataccgcc catagccact cccccattga
     4741 cgtcaatggt ctctatatat ggtctttcct attgacgtca tatgggcggt cctattgacg
     4801 tatatggcgc ctcccccatt gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc
     4861 attgacgtca ataggaccac ccaccattga cgtcaatggg atggctcatt gcccattcat
     4921 atccgttctc acgcccccta ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat
     4981 caatatctat taatagtaac ttggcaagta cattactatt ggaaggacgc cagggtacat
     5041 tggcagtact cccattgacg tcaatggcgg taaatggccc gcgatggctg ccaagtacat
     5101 ccccattgac gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg
     5161 ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg gaac
//
